{"id":15,"date":"2013-06-19T19:29:29","date_gmt":"2013-06-19T19:29:29","guid":{"rendered":"http:\/\/pixlab.cluster006.ovh.net\/clients\/eyeprim\/?page_id=15"},"modified":"2025-05-31T11:13:16","modified_gmt":"2025-05-31T11:13:16","slug":"home","status":"publish","type":"page","link":"https:\/\/www.eyeprim.com\/shop\/","title":{"rendered":"Home"},"content":{"rendered":"

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EYEPRIM™
\nThe first medical device to perform reliable conjunctival
\nimpressions of the living eye\u00a0by OPIA Technologies<\/h4>\n
Contact us<\/a><\/div>\n

[\/vc_column_text][\/vc_column][\/vc_row][vc_row el_class=”maxwidth”][vc_column][vc_tta_accordion style=”modern” color=”orange” c_align=”center” active_section=”18″ collapsible_all=”true”][vc_tta_section title=”What is EYEPRIM™?” tab_id=”1613249909151-09eabccc-6760″][vc_column_text]\"\"EYEPRIM™<\/strong> is a sampling medical device for the collection of conjunctival cells from the ocular surface of the eye, for the purpose of analysis.<\/p>\n

EYEPRIM™<\/strong> is the first validated and reliable tool addressing the sampling method traditionally called impression cytology, or conjunctival impression.<\/p>\n

From an original idea of professor Baudouin (M.D., professor and chair of ophthalmology, Quinze-Vingts Hospital), EYEPRIM™<\/strong> solves all the issues related to this sampling technique and provides great sampling performances, in a reliable, reproducible, and user-independent manner.<\/p>\n

Very easy to use, EYEPRIM™<\/strong> is provided sterile and does not need local anaesthesia.<\/p>\n

EYEPRIM™<\/strong> is certified CE.[\/vc_column_text][vc_column_text el_class=”note”]Consult instructions for use. EYEPRIM™ is a Class I (sterile) medical device according to the directive 93\/42\/CEE, CE 0051 (IMQ)<\/em>[\/vc_column_text][\/vc_tta_section][vc_tta_section title=”How to use” tab_id=”1613249909171-72823ed5-47d2″][vc_row_inner css=”.vc_custom_1535378798656{background-color: #ffffff !important;}”][vc_column_inner css=”.vc_custom_1535378812797{background-color: #ffffff !important;}”][vc_column_text]\"\"[\/vc_column_text][vc_column_text]1. Patient preparation:\u00a0The patient must look up or down according to the selected sampling area.<\/em>
\n No Anaesthesia required.<\/em>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner width=”1\/2″][vc_column_text]\"\"[\/vc_column_text][vc_column_text]2. Position the device upon the conjunctiva. The inner radius can be positioned relative to the limbus.(*)<\/sup> The membrane is stretched onto its support before to be applied on the eyeball.(**)<\/sup><\/em>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/2″][vc_column_text]\"\"[\/vc_column_text][vc_column_text]3. Press gently on the push-button until reaching the stop. Hold from 2 to 3 seconds.<\/em>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner width=”1\/2″][vc_column_text]\"\"[\/vc_column_text][vc_column_text]4. Release the pressure before removing the device from the eye, in order to protect the sample from environment.<\/em>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/2″][vc_column_text]\"\"[\/vc_column_text][vc_column_text]5. Eject the membrane in a storage container by pushing hard onto the push-button.<\/em>
\n (Inner diameter of the storage container should be at least 2 cm.)<\/em>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_column_text el_class=”note”]<\/p>\n

Download Instruction for use (PDF)<\/a><\/p>\n

Consult instructions for use. EYEPRIM™<\/strong> is a Class I (sterile) medical device according to the directive 93\/42\/CEE, CE 0051 (IMQ)[\/vc_column_text][\/vc_tta_section][vc_tta_section title=”Who is using it ?” tab_id=”1613250235537-cc35b5a3-2d2c”][vc_row_inner][vc_column_inner width=”1\/2″][vc_column_text]EYEPRIM™<\/strong> is used by any organization \u2013 Contract Research Organizations, academic or private research centres, pharmaceutical companies \u2013 interested in improving the bio-molecular analysis of the ocular surface with a reliable sampling tool.<\/p>\n

EYEPRIM™<\/strong> is also used in routine clinical practice by ophthalmologists to sample the conjunctiva of patients in order to refine the diagnosis of their ocular surface with further analysis.[\/vc_column_text][vc_column_text el_class=”note”]Consult instructions for use. EYEPRIM™ is a Class I (sterile) medical device according to the directive 93\/42\/CEE, CE 0051 (IMQ)<\/em>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/2″][vc_column_text]\"\"[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_tta_section][vc_tta_section title=”Downstream analysis” tab_id=”1613250425302-738d2306-8f8c”][vc_row_inner][vc_column_inner][vc_column_text]Cytology<\/strong><\/p>\n

Classical Cytology analysis is possible with a suitable microscope after proper staining of the membrane. EYEPRIM™<\/strong>\u00a0sampling membrane is compatible with most staining techniques.<\/p>\n

Note: the membrane cannot be made transparent but powerful transmitted light can go through the membrane. You can also use reflected light microscopy.[\/vc_column_text][vc_column_text]

\"\"
Visualisation of mucins and epithelial cells on conjunctival impression specimen collected with EYEPRIM™ on a healthy subject. Picture from ARVO poster 2014, Colligris et al.<\/figcaption><\/figure>[\/vc_column_text][vc_column_text]Flow cytometry<\/strong><\/p>\n

Cells collected with EYEPRIM™<\/strong> can be analysed by flow cytometry once the cells have been detached from the membrane. A single EYEPRIM™<\/strong> membrane is sufficient for such measurement.[\/vc_column_text][vc_column_text]

\"\"
Measurement of HLA-DR by flow cytometry on conjunctival cells sampled with EYEPRIM™.<\/figcaption><\/figure>[\/vc_column_text][vc_column_text]Quantitative RT-PCR<\/strong><\/p>\n

EYEPRIM™<\/strong> has especially great yield performances for ribonucleic acids (RNA) and allows the measurement of several biomarkers from a single membrane.[\/vc_column_text][vc_column_text]Protein extraction<\/strong><\/p>\n

EYEPRIM™<\/strong>\u00a0provides great protein recovery performances. Particular attention should be paid on the choice of extraction buffer depending on the analysis to be perform. DIGE Buffer is particularly suitable for 2D-PAGE proteomic mapping. Softer buffer should be preferred for protein immunodetection.<\/p>\n

\"\"
Spot detection on proteomic map corresponding to 2D-PAGE of conjunctival tissue obtained using EYEPRIM device. Picture from ARVO poster 2014, Roy et al.<\/figcaption><\/figure>[\/vc_column_text][vc_column_text]3D cytology<\/strong><\/p>\n

3D cytology \u00a0is a patented analysis method (OcuPharm Diagnostics) for the study of goblet cells and goblet cells secretions. This technique is performed on\u00a0EYEPRIM™ <\/strong>conjunctival impression sample, using a Laser Scanning Microscope.<\/p>\n

\"\"
Mucin and its mucin cloud from a muco-deficient (left) and a healthy patient (right). Observation by 3D-Cytology. Pictures from ARVO poster 2014. Colligris et al.<\/figcaption><\/figure>[\/vc_column_text][vc_column_text]<\/p>\n

Download EYEPRIM Handbook (PDF)<\/a><\/p>\n

[\/vc_column_text][vc_column_text el_class=”note”]Consult instructions for use. EYEPRIM™ is a Class I (sterile) medical device according to the directive 93\/42\/CEE, CE 0051 (IMQ)<\/em>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_tta_section][vc_tta_section title=”Performances” tab_id=”1613250461347-331fa14c-994a”][vc_column_text]EYEPRIM™<\/strong> allows very fast, virtually painless, and minimally invasive cell collection from the living eye.<\/p>\n

Technical performances<\/strong><\/h3>\n

[\/vc_column_text][vc_row_inner css=”.vc_custom_1535377807396{background-color: #ffffff !important;}”][vc_column_inner width=”1\/2″][vc_column_text]\"\"[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/2″][vc_column_text el_class=”perfo”]<\/p>\n\n\n\n\n\n\n\n
Precise location of the sampling zone<\/td>\n<\/tr>\n
Immobilization of the eye<\/td>\n<\/tr>\n
Isolation from tear film and eyelid margin<\/td>\n<\/tr>\n
Repetitive sampling parameters (pressure, sampling surface)<\/td>\n<\/tr>\n
Minimize sample contamination<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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Device advantages
\n<\/strong><\/h3>\n